Haematological Cytochemistry

The present status of cytochemistry in the diagnosis of haematological malignancy.

Newer concepts in the application of cytochemistry to the diagnosis of haematological malignancies are discussed. The underlying physiological principles and location of the various enzymes are indicated. Older stains, once thought to be diagnostic, have been shown to be nonspecific. Newer, more accurate stains are now available.

Ultrastructural Cytochemistry

Selective extraction of specific cell components by enzyme or acid hydrolysis is possible from ultrathin sections for electron microscopy and parallel 2 micro sections for light microscopy of tissues fixed in formalin and embedded in a water-soluble polyepoxide, product X133/2097. Normal rat tissues fixed 15 minutes in formalin at 3 degrees C are more rapidly digested by proteinases than those ...

Cytochemistry and Electron Microscopy

THE ALDEHYDES INTRODUCED IN THIS PAPER AND THE MORE APPROPRIATE CONCENTRATIONS FOR THEIR GENERAL USE AS FIXATIVES ARE: 4 to 6.5 per cent glutaraldehyde, 4 per cent glyoxal, 12.5 per cent hydroxyadipaldehyde, 10 per cent crotonaldehyde, 5 per cent pyruvic aldehyde, 10 per cent acetaldehyde, and 5 per cent methacrolein. These were prepared as cacodylate- or phosphate-buffered solutions (0.1 to 0....

Frontiers in Cytochemistry

the names of structures, elements, technics, and numerous subjects widely distributed in this field of knowledge. The first 14 pages are chiefly for the beginner. They are devoted to remarks on laboratory equipment, stains, and choices in method of studying living and dead tissues. For the more experienced the many subjects throughout the book are epitomized for those in haste and supplemented ...

Cytochemistry of leukaemic lymphoid cells.